Journal: PloS one

Article Title: Reduced selenium-binding protein 1 in breast cancer correlates with poor survival and resistance to the anti-proliferative effects of selenium.

doi: 10.1371/journal.pone.0063702

Figure Lengend Snippet: Figure 1. The Expression of SELENBP1 in Normal and Tumor Breast Tissues. Breast cancer tissue arrays were stained by immunohistochemistry using anti-human SELENBP1 antibody at 1:100 dilution. Positive stained cells are shown in dark brown color. (A) Strong positive staining of SELENBP1 in normal breast tissue under low power view (200X). (B–C) Weak positive to negative staining of SELENBP1 in breast cancer tissues under high power view (400X). (D) The Allred scoring distributions of SELENBP1 expression in normal and tumor tissue groups. Inside lines represent means and standard deviations. *p,0.05. (E) Statistical results for the difference between normal and tumor tissues as analyzed by Kruskal-Wallis test. doi:10.1371/journal.pone.0063702.g001

Article Snippet: Breast Cancer Tissue Array Slides Three sets of breast cancer tissue arrays (including normal, primary tumor, and metastases) were obtained from Imgenex or Biomax Inc.

Techniques: Expressing, Staining, Immunohistochemistry, Negative Staining

Journal: PloS one

Article Title: Reduced selenium-binding protein 1 in breast cancer correlates with poor survival and resistance to the anti-proliferative effects of selenium.

doi: 10.1371/journal.pone.0063702

Figure Lengend Snippet: Figure 2. SELENBP1 Expression is Progressively Reduced in Advancing Clinical Stages in Breast Cancer Tissues. (A) The scoring distributions of SELENBP1 expression in normal tissues and tumor tissues at stage II and stage III. Inside lines represent means and standard deviations. **p,0.01. (B) Statistical results for the difference between normal and tumor tissues as analyzed by Kruskal-Wallis test. (C) Survival curves of breast cancer patients with respect to different SELENBP1 expression levels are shown at stage II and (D) stage III. Blue and red lines represent the SELENBP1-high and SELENBP1-low groups, respectively. doi:10.1371/journal.pone.0063702.g002

Article Snippet: Breast Cancer Tissue Array Slides Three sets of breast cancer tissue arrays (including normal, primary tumor, and metastases) were obtained from Imgenex or Biomax Inc.

Techniques: Expressing

Journal: PloS one

Article Title: Reduced selenium-binding protein 1 in breast cancer correlates with poor survival and resistance to the anti-proliferative effects of selenium.

doi: 10.1371/journal.pone.0063702

Figure Lengend Snippet: Figure 3. The Correlation of SELENBP1 Expression with ER, PR, and TP53 in Breast Cancer Tissues. (A) The scoring distributions of SELENBP1 expression in normal tissues and tumor tissues with ER+ and ER– status. The inside lines represent means and standard deviations. **p,0.01. The difference between normal and ER+ and ER– tumor tissues was analyzed by Kruskal-Wallis test and statistical results are shown (B). Survival curves of breast cancer patients with respect to different SELENBP1 expression are shown in ER+ group in (C). The blue line is the SELENBP1- high group and the red line is the SELENBP1-low group. The scoring distributions of SELENBP1 expression in normal and tumor tissues with PR+/PR–

Article Snippet: Breast Cancer Tissue Array Slides Three sets of breast cancer tissue arrays (including normal, primary tumor, and metastases) were obtained from Imgenex or Biomax Inc.

Techniques: Expressing

Journal: JCI Insight

Article Title: Metalloproteinase PAPP-A regulation of IGF-1 contributes to polycystic kidney disease pathogenesis

doi: 10.1172/jci.insight.135700

Figure Lengend Snippet: (A) Relative mRNA expression of IGF-1 pathway components in kidneys of 7.5-month-old C57BL/6J (n = 4–6) and Pkd1RC/RC mice (n = 5–7). PCR data are expressed relative to Gapdh. (B) Correlation between kidney size (total kidney weight relative to heart weight) and renal Pappa mRNA expression in Pkd1RC/RC mice (n = 15). (C) Pappa mRNA levels in various tissues of WT (n = 3–5) and Pkd1RC/RC mice (n = 4–6). (D) Pappa mRNA levels in WT (n = 6) and Pkd2WS25/– (n = 5) mouse kidneys (16 weeks old). (E) ELISA analysis of PAPP-A protein levels in human ADPKD cystic fluid (n = 6) compared with normal serum reference. (F) Immunolocalization of PAPP-A in normal and ADPKD human kidneys. (G) Western blot analysis of PAPP-A protein levels in normal human RCTE and ADPKD cystic epithelial cells (9-12); graph shows quantification relative to tubulin. Scale bars: 200 μm. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by 2-tailed (for Igf1, 1-tailed) Student’s t test.

Article Snippet: Human ADPKD kidney tissue slides were produced in-house under an institutionally approved IRB protocol, and normal human kidney tissue slides were purchased from Novus Biologicals.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Cancer science

Article Title: Coordinate suppression of Sdpr and Fhl1 expression in tumors of the breast, kidney, and prostate.

doi: 10.1111/j.1349-7006.2008.00816.x

Figure Lengend Snippet: Fig. 1. Fhl1 and Sdpr expression in normal epi- thelium and tumors from human breast, kidney, and prostate. Immunohistochemical staining was performed on normal and tumor human tissue. Fhl1 and Sdpr staining was evident in normal epithelial cells of the breast, kidney, and prostate. In contrast, both Fhl1 and Sdpr expression were suppressed in infiltrating mammary duct carcinoma, renal cell carcinoma, and prostate adenocarcinoma. (bar = 60 microns).

Article Snippet: Slides containing 15 infiltrating duct carcinoma specimens with normal adjacent tissue from breast cancer patients (IMH-371, Imgenex), nine prostate adenocarcinoma specimens with adjacent normal tissue and an additional unmatched 41 prostate adenocarcinoma specimens (IMH-303, Imgenex), and nine renal cell carcinoma specimens with adjacent normal tissue and an additional unmatched 31 cell carcinoma specimens (IMH-313, Imgenex) were examined by immunohistochemistry as previously described. (19) Briefly, after removal of paraffin with xylene and rehydration through a graded series of decreasing concentrations of ethanol, protein antigenicity was enhanced by microwaving sections in Target Buffer (Dako, Carpenturia, CA, USA) for 2 min.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Cell Death Discovery

Article Title: Enhanced apoptosis as a possible mechanism to self-limit SARS-CoV-2 replication in porcine primary respiratory epithelial cells in contrast to human cells

doi: 10.1038/s41420-021-00781-w

Figure Lengend Snippet: A – D Formalin-fixed paraffin-embedded cross-sections of human and pig tracheal sections stained for ACE2 with ImmPRESS VR anti-mouse IgG horseradish peroxidase (HRP) polymer detection kit (MP-6402-15). Brown spots represent the presence of ACE2 receptor that interacted with specific antibody is considered a positive expression, and the nucleus counterstained with hematoxylin is blue. Expression of ACE2 in human ( A ) and pig ( C ) trachea revealed by immunostaining with mouse anti-ACE2 monoclonal antibody (4 μg/mL; sc-390851). Note the focal expression of ACE2 on the epithelial cells of pig trachea ( C ; arrow in inset image). B , D Corresponding negative control tissues sections stained with secondary anti-mouse HRP antibody only. Scale bar-100 μm (inset 25 μm). Pig ( n = 3); human ( n = 1). Microscopic morphology of primary human ( E ; HRECs) and pig ( F ; PRECs) respiratory epithelial cells. G Determination of pan-cytokeratin and ACE2 in HRECs and PRECs using flow cytometry. Cells stained with LIVE/DEAD ® Fixable Near-IR Dead Cell Stain Kit, and respective mouse monoclonal antibodies (epithelial cell marker pan-cytokeratin; 0.5 μg/mL; MCA1907T); (ACE2; 4 μg/mL; sc-390851,) incubated for 30 min followed by incubation with Alexa Fluor ® 647 Goat anti-mouse (15 μg/mL) for another 30 min. Data was collected using an Attune NxT flow cytometer. A representative of 10,000 events were acquired and analyzed for each sample. Cells were gated for singlet population using forward (FSA) and side-scatter (SSA) properties, and the mean of percent live cell population was used to quantify the levels of pan-cytokeratin and ACE2 ( n = 4). The bar graph represents the mean (SD).

Article Snippet: Normal human trachea tissue slides were commercially bought (Novus, NBP2-77809 Novus Biologicals, LLC, Centennial, CO, USA), while pig trachea sections were collected for this study.

Techniques: Formalin-fixed Paraffin-Embedded, Staining, Polymer, Expressing, Immunostaining, Negative Control, Flow Cytometry, Marker, Incubation

Journal: Cell Death Discovery

Article Title: Enhanced apoptosis as a possible mechanism to self-limit SARS-CoV-2 replication in porcine primary respiratory epithelial cells in contrast to human cells

doi: 10.1038/s41420-021-00781-w

Figure Lengend Snippet: The tissues and cells presented in this panel were stained with ImmPRESS VR anti-rabbit IgG horseradish peroxidase (HRP) polymer detection kit (MP-6401-15) with a rabbit monoclonal antibody specific for anti-SARS Coronavirus/SARS-Related Coronavirus 2 nucleocapsid (N) protein (0.75 μg/mL) (the following reagent was obtained through BEI Resources, NR-53791). Dark brown spots represent a positive expression of viral N protein, and pale brown represents background staining, while the nucleus counterstained with hematoxylin is blue. A – D Representative images showing SARS-CoV-2 binding in formalin-fixed paraffin-embedded cross-sections of A human and B pig trachea after overnight incubation with 250 μL heat-inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020 (the following reagent was obtained through BEI Resources; NR-52286). C Human ( n = 1) and D pig ( n = 3) tracheal epithelial sections were stained with secondary anti-rabbit HRP antibody only. E – P Detection of SARS-CoV-2 infection in HRECs and PRECs inoculated with SARS-CoV-2, Isolate USA-WA1/2020 at 120 hpi. Paraformaldehyde (4%) fixed cells were stained for SARS-CoV-2 N protein. E , I , and M HRECs and F , J , and N PRECS were inoculated with a viral dose of MOI 5.0 or culture medium, as negative control ( G , K , and O for HRECs; H , L , and P for PRECs); ( n = 6). Scale bar—100 μm.

Article Snippet: Normal human trachea tissue slides were commercially bought (Novus, NBP2-77809 Novus Biologicals, LLC, Centennial, CO, USA), while pig trachea sections were collected for this study.

Techniques: Staining, Polymer, Expressing, Binding Assay, Formalin-fixed Paraffin-Embedded, Incubation, Infection, Negative Control