Journal: PloS one

Article Title: Reduced selenium-binding protein 1 in breast cancer correlates with poor survival and resistance to the anti-proliferative effects of selenium.

doi: 10.1371/journal.pone.0063702

Figure Lengend Snippet: Figure 1. The Expression of SELENBP1 in Normal and Tumor Breast Tissues. Breast cancer tissue arrays were stained by immunohistochemistry using anti-human SELENBP1 antibody at 1:100 dilution. Positive stained cells are shown in dark brown color. (A) Strong positive staining of SELENBP1 in normal breast tissue under low power view (200X). (B–C) Weak positive to negative staining of SELENBP1 in breast cancer tissues under high power view (400X). (D) The Allred scoring distributions of SELENBP1 expression in normal and tumor tissue groups. Inside lines represent means and standard deviations. *p,0.05. (E) Statistical results for the difference between normal and tumor tissues as analyzed by Kruskal-Wallis test. doi:10.1371/journal.pone.0063702.g001

Article Snippet: Breast Cancer Tissue Array Slides Three sets of breast cancer tissue arrays (including normal, primary tumor, and metastases) were obtained from Imgenex or Biomax Inc.

Techniques: Expressing, Staining, Immunohistochemistry, Negative Staining

Journal: PloS one

Article Title: Reduced selenium-binding protein 1 in breast cancer correlates with poor survival and resistance to the anti-proliferative effects of selenium.

doi: 10.1371/journal.pone.0063702

Figure Lengend Snippet: Figure 2. SELENBP1 Expression is Progressively Reduced in Advancing Clinical Stages in Breast Cancer Tissues. (A) The scoring distributions of SELENBP1 expression in normal tissues and tumor tissues at stage II and stage III. Inside lines represent means and standard deviations. **p,0.01. (B) Statistical results for the difference between normal and tumor tissues as analyzed by Kruskal-Wallis test. (C) Survival curves of breast cancer patients with respect to different SELENBP1 expression levels are shown at stage II and (D) stage III. Blue and red lines represent the SELENBP1-high and SELENBP1-low groups, respectively. doi:10.1371/journal.pone.0063702.g002

Article Snippet: Breast Cancer Tissue Array Slides Three sets of breast cancer tissue arrays (including normal, primary tumor, and metastases) were obtained from Imgenex or Biomax Inc.

Techniques: Expressing

Journal: PloS one

Article Title: Reduced selenium-binding protein 1 in breast cancer correlates with poor survival and resistance to the anti-proliferative effects of selenium.

doi: 10.1371/journal.pone.0063702

Figure Lengend Snippet: Figure 3. The Correlation of SELENBP1 Expression with ER, PR, and TP53 in Breast Cancer Tissues. (A) The scoring distributions of SELENBP1 expression in normal tissues and tumor tissues with ER+ and ER– status. The inside lines represent means and standard deviations. **p,0.01. The difference between normal and ER+ and ER– tumor tissues was analyzed by Kruskal-Wallis test and statistical results are shown (B). Survival curves of breast cancer patients with respect to different SELENBP1 expression are shown in ER+ group in (C). The blue line is the SELENBP1- high group and the red line is the SELENBP1-low group. The scoring distributions of SELENBP1 expression in normal and tumor tissues with PR+/PR–

Article Snippet: Breast Cancer Tissue Array Slides Three sets of breast cancer tissue arrays (including normal, primary tumor, and metastases) were obtained from Imgenex or Biomax Inc.

Techniques: Expressing

Journal: iScience

Article Title: Pulmonary surfactants and the respiratory-renal connection in steroid-sensitive nephrotic syndrome of childhood

doi: 10.1016/j.isci.2022.104694

Figure Lengend Snippet: Surfactant levels and urinary protein in SSNS/MCD (A) Plasma SP-A in control and ten subjects with SSNS/MCD in consecutive relapse/remission cycles (from 2 to 4 cycles). (B) Plasma SP-D in control and ten subjects with SSNS/MCD in consecutive relapse/remission cycles (from 2 to 4 cycles). (C) Correlation analysis between plasma SP-A and urinary SIRPα levels in samples from two controls (black symbols) and ten subjects with SSNS/MCD in relapse (blue symbols) and remission (red symbols). (D) Correlation analysis between plasma SP-A and urinary protein in samples from two controls (black symbols) and ten subjects with SSNS/MCD in relapse (blue symbols) and remission (red symbols). (E) Representative western blot for SP-A in the urine of a control subject (C1) and three patients with MCD in relapse (Rl1 to Rl3) and remission (Rm1 to Rm3). Statistical Analysis: One-Way ANOVA for A and B. For (C and D) Pearson r correlation analysis.

Article Snippet: Mouse SP-A (SFTPA) ELISA detection kit , Novus Biologicals , NBP2-77693.

Techniques: Clinical Proteomics, Control, Western Blot

Journal: iScience

Article Title: Pulmonary surfactants and the respiratory-renal connection in steroid-sensitive nephrotic syndrome of childhood

doi: 10.1016/j.isci.2022.104694

Figure Lengend Snippet: Intranasal instillation of LPS and PolyI:C and proteinuria in mice (A) Schematic depicting the protocol to induce proteinuria. Saline (red) or a combination of LPS (80 μg) and Poly I:C (240 μg) (blue) is administered intranasally once daily for three consecutive days. Urine is collected every 6 h and 3 h after the fourth injection, plasma, lung, and kidney tissue are collected for analysis. (B) Plasma SP-A levels in unmanipulated (black) mice or receiving either saline (red) or LPS/Poly I:C combination (n = 5 mice per group). (C) Representative confocal images for SP-A (green) and synaptopodin (red) colocalization in kidney glomeruli of mice receiving saline or LPS/Poly I:C. Merged colocalization is shown in yellow. Size bar: 200 μm. Images are representative from n = 5 mice per group (D) Confocal analysis of SIRPα (green) and SP-A (red) colocalization in kidney glomeruli of mice receiving saline or LPS/Poly I:C. Merged colocalization is shown in yellow. Size bar: 200 μm. Images are representative from five mice. (E) Co-immunoprecipitation of SIRPα and detection of SP-A in kidney cortex extracts of unmanipulated, saline-exposed, and LPS/Poly I:C-exposed mice. Total input (500 ug) and amount retained in preclearance is shown in lanes one and two; n = 3 mice per group. (F) Six hour albumin excretion over the 72-h period in unmanipulated (black) mice receiving either saline or LPS/Poly I:C. (G) 72-h accumulated albumin excretion in the urine of the same mice in F). n = 5 mice per group. (H) Representative electron microscopy podocyte images from saline- or LPS/PolyI:C-treated mice for 24 h. Blue arrows represent areas of foot effacement. Statistical Analysis: One-Way ANOVA, Tukey post hoc analysis for (B) and (G), data are represented as mean ± SEM.

Article Snippet: Mouse SP-A (SFTPA) ELISA detection kit , Novus Biologicals , NBP2-77693.

Techniques: Saline, Injection, Clinical Proteomics, Immunoprecipitation, Electron Microscopy

Journal: iScience

Article Title: Pulmonary surfactants and the respiratory-renal connection in steroid-sensitive nephrotic syndrome of childhood

doi: 10.1016/j.isci.2022.104694

Figure Lengend Snippet:

Article Snippet: Mouse SP-A (SFTPA) ELISA detection kit , Novus Biologicals , NBP2-77693.

Techniques: Recombinant, Protease Inhibitor, Enzyme-linked Immunosorbent Assay